Human MAT2A ELISA Kit

Product Name:Human MAT2A ELISA Kit
Packing:96T

Catalog No.:ELA-E12626h

Gene Name:MAT2A

Detect Range:0.156-10 ng/mL

Sensitivity:0.15ng/ml

Target Protein Name:Human S-adenosylmethionine synthase isoform type-2

Alternative Name:S-adenosylmethionine synthase isoform type-2, AdoMet synthase 2, Methionine adenosyltransferase 2, MAT 2, Methionine adenosyltransferase II, MAT-II, MAT2A, AMS2, MATA2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human MAT2A ELISA Kit allows for the in vitro quantitative determination of Human S-adenosylmethionine synthase isoform type-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human MAT2A ELISA Kit has been pre-coated with an MAT2A antibody specific to Human S-adenosylmethionine synthase isoform type-2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human S-adenosylmethionine synthase isoform type-2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human S-adenosylmethionine synthase isoform type-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human S-adenosylmethionine synthase isoform type-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!