Human MCM2 ELISA Kit

Product Name:Human MCM2 ELISA Kit
Packing:96T

Catalog No.:ELA-E1768h

Gene Name:MCM2

Detect Range:0.078-5 ng/mL

Sensitivity:0.07ng/mL

Target Protein Name:Human DNA replication licensing factor MCM2

Alternative Name:DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, BM28, CCNL1, CDCL1, KIAA0030
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human MCM2 ELISA Kit allows for the in vitro quantitative determination of Human DNA replication licensing factor MCM2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human MCM2 ELISA Kit has been pre-coated with an MCM2 antibody specific to Human DNA replication licensing factor MCM2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human DNA replication licensing factor MCM2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human DNA replication licensing factor MCM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human DNA replication licensing factor MCM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!