Human MEP1A ELISA Kit

Product Name:Human MEP1A ELISA Kit
Packing:96T

Catalog No.:ELA-E2226h

Gene Name:MEP1A

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Human Meprin A subunit alpha

Alternative Name:Meprin A subunit alpha, Endopeptidase-2, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, PABA peptide hydrolase, PPH alpha, MEP1A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human MEP1A ELISA Kit allows for the in vitro quantitative determination of Human Meprin A subunit alpha concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human MEP1A ELISA Kit has been pre-coated with an MEP1A antibody specific to Human Meprin A subunit alpha .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Meprin A subunit alpha and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Meprin A subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Meprin A subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!