Human Midnolin, MIDN ELISA KIT
Product Name:Human Midnolin, MIDN ELISA KIT
Packing:96T
Catalog No.:ELI-20373h
Gene Name:MIDN
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Midnolin(MIDN
Alternative Name:MIDN,Midnolin(MIDN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Midnolin, MIDN ELISA KIT allows for the in vitro quantitative determination of Midnolin(MIDN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Midnolin, MIDN ELISA KIT has been pre-coated with an Midnolin(MIDN antibody specific to Midnolin(MIDN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Midnolin(MIDN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Midnolin(MIDN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Midnolin(MIDN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!