Human Mitochondrial Rho GTPase 1, RHOT1 ELISA KIT
Product Name:Human Mitochondrial Rho GTPase 1, RHOT1 ELISA KIT
Packing:96T
Catalog No.:ELI-38968h
Gene Name:RHOT1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Mitochondrial Rho GTPase 1(RHOT1
Alternative Name:RHOT1,Mitochondrial Rho GTPase 1(RHOT1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Mitochondrial Rho GTPase 1, RHOT1 ELISA KIT allows for the in vitro quantitative determination of Mitochondrial Rho GTPase 1(RHOT1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Mitochondrial Rho GTPase 1, RHOT1 ELISA KIT has been pre-coated with an Mitochondrial Rho GTPase 1(RHOT1 antibody specific to Mitochondrial Rho GTPase 1(RHOT1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mitochondrial Rho GTPase 1(RHOT1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mitochondrial Rho GTPase 1(RHOT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mitochondrial Rho GTPase 1(RHOT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!