Human mRNA- decapping enzyme 1A, DCP1A ELISA KIT
Product Name:Human mRNA- decapping enzyme 1A, DCP1A ELISA KIT
Packing:96T
Catalog No.:ELI-47012h
Gene Name:DCP1A
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:mRNA-decapping enzyme 1A(DCP1A
Alternative Name:DCP1A,mRNA-decapping enzyme 1A(DCP1A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human mRNA- decapping enzyme 1A, DCP1A ELISA KIT allows for the in vitro quantitative determination of mRNA-decapping enzyme 1A(DCP1A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human mRNA- decapping enzyme 1A, DCP1A ELISA KIT has been pre-coated with an mRNA-decapping enzyme 1A(DCP1A antibody specific to mRNA-decapping enzyme 1A(DCP1A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for mRNA-decapping enzyme 1A(DCP1A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain mRNA-decapping enzyme 1A(DCP1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of mRNA-decapping enzyme 1A(DCP1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!