Human MSH3 ELISA KIT

Catalog  EF000955

Packing 48T/96T                     Manual

Reactivity Human

Detect Range 0.156-10ng/ml

MSH3 ELISA KIT Sensitivity< 0.1ng/ml

Application Human MSH3 ELISA KIT  is for detecting the concentration of Human MSH3 in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of Human MSH3 ELISA KIT

The microtiter plate provided in Human MSH3 ELISA KIT has been pre-coated with an Human MSH3 antibody specific to Human MSH3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human MSH3antibody preparation specific for Human MSH3 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human MSH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human MSH3in the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that Human MSH3 ELISA KIT is only for RUO (Research Use Only), Not for the diagnostic Purpose!