Human Monofunctional C1- tetrahydrofolate synthase mitochondrial, MTHFD1L ELISA KIT 96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Monofunctional C1-tetrahydrofolate synthase mitochondrial,Formyltetrahydrofolate synthetase,C1TM, mitochondrial C1 tetrahydrofolate synthase,FTHFSDC1; MTC1THFS; dJ292B18.2; 10-formyl-THF synthetase; formyltetrahydrofolate synthetase domain containing 1; methylenetetrahydrofolate dehydrogenase (NADP+ dependent)1-like

Search name

Human Monofunctional C1-tetrahydrofolate synthase mitochondrial ELISA KIT ,Human Formyltetrahydrofolate synthetase ELISA KIT ,Human C1TM ELISA KIT ,Human  mitochondrial C1 tetrahydrofolate synthase ELISA KIT ,Human FTHFSDC1 ELISA KIT ,Human MTC1THFS ELISA KIT ,Human dJ292B18.2 ELISA KIT ,Human 10-formyl-THF synthetase ELISA KIT ,Human formyltetrahydrofolate synthetase domain containing 1 ELISA KIT ,Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent)1-like ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human monofunctional c1-tetrahydrofolate synthase, mitochondrial,C1TM concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1TM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C1TM and Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C1TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C1TM in the samples is then determined by comparing the O.D. of the samples to the standard curve.