Human Multidrug resistance-associated protein 4, ABCC4 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ABCC4,MRP4,MOAT-B; MOATB; MRP/cMOAT-related ABC transporter; ATP-binding cassette, sub-family C (CFTR/MRP) member 4; canalicular multispecific organic anion transporter (ABC superfamily); multi-specific organic anion transporter B; ATP-binding cassette sub-family C (CFTR/MRP) member 4,Multi-specific organic anion transporter B,ATP-binding cassette sub-family C member 4,MRP/cMOAT-related ABC transporter, Multidrug resistance-associated protein 4

Search name

Human ABCC4 ELISA KIT ,Human MRP4 ELISA KIT ,Human MOAT-B ELISA KIT ,Human MOATB ELISA KIT ,Human MRP/cMOAT-related ABC transporter ELISA KIT ,Human ATP-binding cassette ELISA KIT ,Human sub-family C (CFTR/MRP) member 4 ELISA KIT ,Human canalicular multispecific organic anion transporter (ABC superfamily) ELISA KIT ,Human multi-specific organic anion transporter B ELISA KIT ,Human ATP-binding cassette sub-family C (CFTR/MRP) member 4 ELISA KIT ,Human Multi-specific organic anion transporter B ELISA KIT ,Human ATP-binding cassette sub-family C member 4 ELISA KIT ,Human MRP/cMOAT-related ABC transporter ELISA KIT ,Human Multidrug resistance-associated protein 4

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human multidrug resistance-associated protein 4,Multidrug resistance-associated protein 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Multidrug resistance-associated protein 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Multidrug resistance-associated protein 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Multidrug resistance-associated protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Multidrug resistance-associated protein 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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