Human muscle and microspikes RAS, Mras ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY
Alternative name
Human MRAS ELISA KIT ,Human M-RAs ELISA KIT ,Human R-RAS3 ELISA KIT ,Human RRAS3 ELISA KIT ,Rar Xras ELISA KIT,Human muscle and microspikes RAS ELISA KIT ,Human ras-related protein R-Ras3 ELISA KIT ,Human muscle RAS oncogene homolog ELISA KIT,Human Ras-related protein M-Ras ELISA KIT ,Human Ras-related protein R-Ras3 ELISA KIT
Intended use
Human Mras ELISA KIT allows for the in vitro quantitative determination of Human Mras concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Mras. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mras and Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Mras, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Mras in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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