Human Myeloblastin, PRTN3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Myeloblastin,Wegener autoantigen,P29,Neutrophil proteinase 4(NP-4),Leukocyte proteinase 3(PR-3,PR3),AGP7,C-ANCA antigen, PRTN3,ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP-4; NP4; P29; PR-3; PR3; C-ANCA antigen; Wegener granulomatosis autoantigen; azurophil granule protein 7; leukocyte proteinase 3; myeloblastin; neutrophil proteinase 4; serine proteinase, neutrophil; wegener autoantigen; proteinase 3

Search name

Human Myeloblastin ELISA KIT ,Human Wegener autoantigen ELISA KIT ,Human P29 ELISA KIT ,Human Neutrophil proteinase 4 ELISA KIT ,Human NP-4 ELISA KIT ,Human Leukocyte proteinase 3 ELISA KIT ,Human PR-3 ELISA KIT ,Human PR3 ELISA KIT ,Human AGP7 ELISA KIT ,Human C-ANCA antigen ELISA KIT ,Human  PRTN3 ELISA KIT ,Human ACPA ELISA KIT ,Human AGP7 ELISA KIT ,Human C-ANCA ELISA KIT ,Human CANCA ELISA KIT ,Human MBN ELISA KIT ,Human MBT ELISA KIT ,Human NP-4 ELISA KIT ,Human NP4 ELISA KIT ,Human P29 ELISA KIT ,Human PR-3 ELISA KIT ,Human PR3 ELISA KIT ,Human C-ANCA antigen ELISA KIT ,Human Wegener granulomatosis autoantigen ELISA KIT ,Human azurophil granule protein 7 ELISA KIT ,Human leukocyte proteinase 3 ELISA KIT ,Human myeloblastin ELISA KIT ,Human neutrophil proteinase 4 ELISA KIT ,Human serine proteinase neutrophil ELISA KIT ,Human wegener autoantigen ELISA KIT ,Human proteinase 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Myeloblastin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloblastin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myeloblastin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myeloblastin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Myeloblastin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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