Human Myosin light chain kinase family member 4, MYLK4 ELISA KIT
Product Name:Human Myosin light chain kinase family member 4, MYLK4 ELISA KIT
Packing:96T
Catalog No.:ELI-39468h
Gene Name:MYLK4
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Myosin light chain kinase family member 4(MYLK4
Alternative Name:MYLK4,Myosin light chain kinase family member 4(MYLK4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Myosin light chain kinase family member 4, MYLK4 ELISA KIT allows for the in vitro quantitative determination of Myosin light chain kinase family member 4(MYLK4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Myosin light chain kinase family member 4, MYLK4 ELISA KIT has been pre-coated with an Myosin light chain kinase family member 4(MYLK4 antibody specific to Myosin light chain kinase family member 4(MYLK4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Myosin light chain kinase family member 4(MYLK4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myosin light chain kinase family member 4(MYLK4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Myosin light chain kinase family member 4(MYLK4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!