Human N- myc proto- oncogene protein, MYCN ELISA KIT
Product Name:Human N- myc proto- oncogene protein, MYCN ELISA KIT
Packing:96T
Catalog No.:ELI-13880h
Gene Name:MYCN
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:N-myc proto-oncogene protein(MYCN
Alternative Name:MYCN,N-myc proto-oncogene protein(MYCN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human N- myc proto- oncogene protein, MYCN ELISA KIT allows for the in vitro quantitative determination of N-myc proto-oncogene protein(MYCN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human N- myc proto- oncogene protein, MYCN ELISA KIT has been pre-coated with an N-myc proto-oncogene protein(MYCN antibody specific to N-myc proto-oncogene protein(MYCN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for N-myc proto-oncogene protein(MYCN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-myc proto-oncogene protein(MYCN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of N-myc proto-oncogene protein(MYCN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!