Human NCOA1 ELISA KIT

Catalog  EF000575

Packing 48T/96T                        Protocol

Reactivity Human

Detect Range  0.156 ng/mL - 10 ng/mL. The standard curve concentrations used for the ELISA’s were 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.31 ng/mL, 0.156 ng/mL, 0 ng/mL.

NCOA1 ELISA KIT Sensitivity< 0.078ng/ml

Application Human NCOA1 ELISA KIT  is for detecting the concentration of Human NCOA1 in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of Human NCOA1 ELISA KIT

The microtiter plate provided in Human NCOA1 ELISA KIT has been pre-coated with an Human NCOA1 antibody specific to Human NCOA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human NCOA1antibody preparation specific for Human NCOA1 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NCOA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human NCOA1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that Human NCOA1 ELISA KIT only for RUO (Research Use Only), Not for the diagnostic Purpose!