Human NDUFS8 ELISA Kit
Product Name:Human NDUFS8 ELISA Kit
Packing:96T
Catalog No.:ELA-E1604h
Gene Name:NDUFS8
Detect Range:0.312-20 ng/mL
Sensitivity:0.1ng/mL
Target Protein Name:Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
Alternative Name:NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, Complex I-23kD, CI-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, NDUFS8, 1.6.5.3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NDUFS8 ELISA Kit allows for the in vitro quantitative determination of Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NDUFS8 ELISA Kit has been pre-coated with an NDUFS8 antibody specific to Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm