Human Negative elongation factor B, COBRA1 ELISA KIT
Product Name:Human Negative elongation factor B, COBRA1 ELISA KIT
Packing:96T
Catalog No.:ELI-13803h
Gene Name:COBRA1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Negative elongation factor B(COBRA1
Alternative Name:COBRA1,Negative elongation factor B(COBRA1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Negative elongation factor B, COBRA1 ELISA KIT allows for the in vitro quantitative determination of Negative elongation factor B(COBRA1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Negative elongation factor B, COBRA1 ELISA KIT has been pre-coated with an Negative elongation factor B(COBRA1 antibody specific to Negative elongation factor B(COBRA1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Negative elongation factor B(COBRA1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Negative elongation factor B(COBRA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Negative elongation factor B(COBRA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!