Human Neuritin 1 ELISA Kit
Product Name:Human Neuritin 1 ELISA Kit
Packing:96T
Catalog No.:ELA-E13381h
Gene Name:NRN1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Neuritin 1
Alternative Name:NRN1,Neuritin 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Neuritin 1 ELISA Kit allows for the in vitro quantitative determination of Neuritin 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Neuritin 1 ELISA Kit has been pre-coated with an Neuritin 1 antibody specific to Neuritin 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Neuritin 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuritin 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Neuritin 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!