Human Neurocan core protein, NCAN ELISA KIT

Product Name:Human Neurocan core protein, NCAN ELISA KIT
Packing:96T

Catalog No.:ELI-13183h

Gene Name:NCAN

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Neurocan core protein(NCAN

Alternative Name:NCAN,Neurocan core protein(NCAN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Neurocan core protein, NCAN ELISA KIT allows for the in vitro quantitative determination of Neurocan core protein(NCAN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Neurocan core protein, NCAN ELISA KIT has been pre-coated with an Neurocan core protein(NCAN antibody specific to Neurocan core protein(NCAN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Neurocan core protein(NCAN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neurocan core protein(NCAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Neurocan core protein(NCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!