Human NF- kappa- B inhibitor alpha, Nfkbia ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NF-kappa-B inhibitor alpha,I-kappa-B-alpha(IkB-alpha,IkappaBalpha),RL/IF-1,Nfkbia,IkappaBalpha,NF-kappa-B inhibitor alpha; IKBA; MAD-3; NFKBI; I-kappa-B-alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

Search name

Human NF-kappa-B inhibitor alpha ELISA KIT ,Human I-kappa-B-alpha ELISA KIT ,Human IkB-alpha ELISA KIT ,Human IkappaBalpha ELISA KIT ,Human RL/IF-1 ELISA KIT ,Human Nfkbia ELISA KIT ,Human IkappaBalpha ELISA KIT ,Human NF-kappa-B inhibitor alpha ELISA KIT ,Human IKBA ELISA KIT ,Human MAD-3 ELISA KIT ,Human NFKBI ELISA KIT ,Human I-kappa-B-alpha ELISA KIT ,Human ikB-alpha ELISA KIT ,Human major histocompatibility complex enhancer-binding protein MAD3 ELISA KIT ,Human nuclear factor of kappa light chain gene enhancer in B-cells ELISA KIT ,Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human NF-kappa-B inhibitor alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-kappa-B inhibitor alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NF-kappa-B inhibitor alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-kappa-B inhibitor alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NF-kappa-B inhibitor alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.