Human NGF ELISA Kit
Product Name:Human NGF ELISA Kit
Packing:96T
Catalog No.:ELA-E0105h
Gene Name:NGF
Detect Range:31.2-2000 pg/mL
Sensitivity:12pg/mL
Target Protein Name:Human Beta-nerve growth factor
Alternative Name:Beta-nerve growth factor, Beta-NGF, NGF, NGFB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NGF ELISA Kit allows for the in vitro quantitative determination of Human Beta-nerve growth factor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NGF ELISA Kit has been pre-coated with an NGF antibody specific to Human Beta-nerve growth factor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Beta-nerve growth factor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Beta-nerve growth factor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm