NIM1K ELISA KIT|Human
Catalog EF005554
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
NIM1K ELISA KIT Sensitivity< 0.094ng/ml
Application NIM1K ELISA KIT|Human is for detecting the concentration of Human NIM1K in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of NIM1K ELISA KIT|Human
The microtiter plate provided in NIM1K ELISA KIT|Human has been pre-coated with an Human NIM1K antibody specific to Human NIM1K. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human NIM1Kantibody preparation specific for Human NIM1K then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NIM1K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human NIM1Kin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that NIM1K ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!