Human NNMT ELISA Kit
Product Name:Human NNMT ELISA Kit
Packing:96T
Catalog No.:ELA-E15042h
Gene Name:NNMT
Detect Range:0.156-10 ng/mL
Sensitivity:0.054ng/ml
Target Protein Name:Human Nicotinamide N-methyltransferase
Alternative Name:Nicotinamide N-methyltransferase, NNMT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NNMT ELISA Kit allows for the in vitro quantitative determination of Human Nicotinamide N-methyltransferase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NNMT ELISA Kit has been pre-coated with an NNMT antibody specific to Human Nicotinamide N-methyltransferase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Nicotinamide N-methyltransferase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Nicotinamide N-methyltransferase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Nicotinamide N-methyltransferase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!