Human Non- receptor tyrosine- protein kinase TNK1, TNK1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Non-receptor tyrosine-protein kinase TNK1,CD38 negative kinase 1,;

Search name

Human TNK1 ELISA KIT ,Human CD38 negative kinase 1 ELISA KIT ,Human tyrosine kinase non-receptor 1 ELISA KIT ,Human  Non-receptor tyrosine-protein kinase TNK1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination ofHuman Non-receptor tyrosine-protein kinase TNK1 concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non-receptor tyrosine-protein kinase TNK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forNon-receptor tyrosine-protein kinase TNK1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Non-receptor tyrosine-protein kinase TNK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Non-receptor tyrosine-protein kinase TNK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF Manual download