Human NT5E ELISA Kit

Product Name:Human NT5E ELISA Kit
Packing:96T

Catalog No.:ELA-E0104h

Gene Name:NT5E

Detect Range:78-5000 pg/mL

Sensitivity:45pg/mL

Target Protein Name:Human 5'-nucleotidase

Alternative Name:5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E, NT5, NTE, CD73
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human NT5E ELISA Kit allows for the in vitro quantitative determination of Human 5'-nucleotidase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human NT5E ELISA Kit has been pre-coated with an NT5E antibody specific to Human 5'-nucleotidase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human 5'-nucleotidase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human 5'-nucleotidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm