Human NTF4 ELISA Kit
Product Name:Human NTF4 ELISA Kit
Packing:96T
Catalog No.:ELA-E0107h
Gene Name:NTF4
Detect Range:93.75-6000 pg/mL
Sensitivity:45pg/mL
Target Protein Name:Human Neurotrophin-4
Alternative Name:Neurotrophin-4, NT-4, Neurotrophin-5, NT-5, Neutrophic factor 4, NTF4, NTF5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NTF4 ELISA Kit allows for the in vitro quantitative determination of Human Neurotrophin-4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NTF4 ELISA Kit has been pre-coated with an NTF4 antibody specific to Human Neurotrophin-4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Neurotrophin-4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Neurotrophin-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm