Human OGG1 ELISA Kit
Product Name:Human OGG1 ELISA Kit
Packing:96T
Catalog No.:ELA-E1437h
Gene Name:OGG1
Detect Range:0.156-10 ng/mL
Sensitivity:0.45ng/mL
Target Protein Name:Human N-glycosylase/DNA lyase
Alternative Name:N-glycosylase/DNA lyase, OGG1, MMH, MUTM, OGH1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human OGG1 ELISA Kit allows for the in vitro quantitative determination of Human N-glycosylase/DNA lyase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human OGG1 ELISA Kit has been pre-coated with an OGG1 antibody specific to Human N-glycosylase/DNA lyase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human N-glycosylase/DNA lyase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human N-glycosylase/DNA lyase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm