Human Origin recognition complex subunit 3, ORC3 ELISA KIT
Product Name:Human Origin recognition complex subunit 3, ORC3 ELISA KIT
Packing:96T
Catalog No.:ELI-12544h
Gene Name:ORC3
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Origin recognition complex subunit 3(ORC3
Alternative Name:ORC3,Origin recognition complex subunit 3(ORC3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Origin recognition complex subunit 3, ORC3 ELISA KIT allows for the in vitro quantitative determination of Origin recognition complex subunit 3(ORC3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Origin recognition complex subunit 3, ORC3 ELISA KIT has been pre-coated with an Origin recognition complex subunit 3(ORC3 antibody specific to Origin recognition complex subunit 3(ORC3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Origin recognition complex subunit 3(ORC3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Origin recognition complex subunit 3(ORC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Origin recognition complex subunit 3(ORC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!