Human OSM ELISA Kit

Product Name:Human OSM ELISA Kit
Packing:96T

Catalog No.:ELA-E0110h

Gene Name:OSM

Detect Range:0.156-10 ng/mL

Sensitivity:0.054ng/mL

Target Protein Name:Human Oncostatin-M

Alternative Name:Oncostatin-M, OSM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human OSM ELISA Kit allows for the in vitro quantitative determination of Human Oncostatin-M concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human OSM ELISA Kit has been pre-coated with an OSM antibody specific to Human Oncostatin-M .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Oncostatin-M and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Oncostatin-M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm