Human Otolin- 1, OTOL1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
OTOL1,C1QTNF15; C1q and tumor necrosis factor related protein 15; otolin 1 homolog; otolin-1; otolin 1
Search name
Human OTOL1 ELISA KIT ,Human C1QTNF15 ELISA KIT ,Human C1q and tumor necrosis factor related protein 15 ELISA KIT ,Human otolin 1 homolog ELISA KIT ,Human otolin-1 ELISA KIT ,Human otolin 1 ELISA KIT
Intended use
Human OTOL1 ELISA KIT allows for the in vitro quantitative determination of Human Otolin- 1, OTOL1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human OTOL1 ELISA KIT has been pre-coated with an antibody specific to Otolin- 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Otolin- 1 and Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Otolin- 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Otolin- 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human OTOL1 ELISA KIT Range 15.6-1000pg/mL
OTOL1 Related Products
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Anti OTOL1 Antidody