Human Pappalysin- 1, PAPPA ELISA KIT

Product Name:Human Pappalysin- 1, PAPPA ELISA KIT
Packing:96T

Catalog No.:ELI-02712h

Gene Name:PAPPA

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Pappalysin-1(PAPPA

Alternative Name:PAPPA,Pappalysin-1(PAPPA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Pappalysin- 1, PAPPA ELISA KIT allows for the in vitro quantitative determination of Pappalysin-1(PAPPA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Pappalysin- 1, PAPPA ELISA KIT has been pre-coated with an Pappalysin-1(PAPPA antibody specific to Pappalysin-1(PAPPA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Pappalysin-1(PAPPA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pappalysin-1(PAPPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Pappalysin-1(PAPPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!