Human PCK1 ELISA Kit
Product Name:Human PCK1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0936h
Gene Name:PCK1
Detect Range:0.312-20 ng/mL
Sensitivity:0.12ng/mL
Target Protein Name:Human Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Alternative Name:Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, Phosphoenolpyruvate carboxylase, PCK1, PEPCK1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human PCK1 ELISA Kit allows for the in vitro quantitative determination of Human Phosphoenolpyruvate carboxykinase, cytosolic [GTP] concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human PCK1 ELISA Kit has been pre-coated with an PCK1 antibody specific to Human Phosphoenolpyruvate carboxykinase, cytosolic [GTP] .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Phosphoenolpyruvate carboxykinase, cytosolic [GTP] and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Phosphoenolpyruvate carboxykinase, cytosolic [GTP], biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm