Human PCK2 ELISA Kit

Product Name:Human PCK2 ELISA Kit
Packing:96T

Catalog No.:ELA-E10365h

Gene Name:PCK2

Detect Range:0.156-10 ng/mL

Sensitivity:0.098ng/mL

Target Protein Name:Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Alternative Name:Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, Phosphoenolpyruvate carboxylase, PCK2, PEPCK2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PCK2 ELISA Kit allows for the in vitro quantitative determination of Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PCK2 ELISA Kit has been pre-coated with an PCK2 antibody specific to Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!