Human PDGFA ELISA Kit

Product Name:Human PDGFA ELISA Kit
Packing:96T

Catalog No.:ELA-E0112h

Gene Name:PDGFA

Detect Range:31.2-2000 pg/mL

Sensitivity:12pg/mL

Target Protein Name:Human Platelet-derived growth factor subunit A

Alternative Name:Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGFA, PDGF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PDGFA ELISA Kit allows for the in vitro quantitative determination of Human Platelet-derived growth factor subunit A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PDGFA ELISA Kit has been pre-coated with an PDGFA antibody specific to Human Platelet-derived growth factor subunit A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Platelet-derived growth factor subunit A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Platelet-derived growth factor subunit A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm