Human PDGFB ELISA Kit

Product Name:Human PDGFB ELISA Kit
Packing:96T

Catalog No.:ELA-E0633h

Gene Name:PDGFB

Detect Range:31.2-2000 pg/mL

Sensitivity:15pg/mL

Target Protein Name:Human Platelet-derived growth factor subunit B

Alternative Name:Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, PDGFB, PDGF2,
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PDGFB ELISA Kit allows for the in vitro quantitative determination of Human Platelet-derived growth factor subunit B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PDGFB ELISA Kit has been pre-coated with an PDGFB antibody specific to Human Platelet-derived growth factor subunit B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Platelet-derived growth factor subunit B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Platelet-derived growth factor subunit B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm