Human PDGFRB ELISA Kit
Product Name:Human PDGFRB ELISA Kit
Packing:96T
Catalog No.:ELA-E2061h
Gene Name:PDGFRB
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Human Beta-type platelet-derived growth factor receptor
Alternative Name:Beta-type platelet-derived growth factor receptor, PDGF-R-beta, CD140 antigen-like family member B, PDGFRB, 2.7.10.1, Beta-type platelet-derived growth factor receptor, CD140 antigen-like
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human PDGFRB ELISA Kit allows for the in vitro quantitative determination of Human Beta-type platelet-derived growth factor receptor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human PDGFRB ELISA Kit has been pre-coated with an PDGFRB antibody specific to Human Beta-type platelet-derived growth factor receptor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Beta-type platelet-derived growth factor receptor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Beta-type platelet-derived growth factor receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Beta-type platelet-derived growth factor receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!