Human PDXP ELISA Kit

Product Name:Human PDXP ELISA Kit
Packing:96T

Catalog No.:ELA-E1712h

Gene Name:PDXP

Detect Range:0.625-40 ng/mL

Sensitivity:0.176 ng/mL

Target Protein Name:Human Pyridoxal phosphate phosphatase

Alternative Name:Pyridoxal phosphate phosphatase, PLP phosphatase, Chronophin, PDXP, CIN, PLP, PLPP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PDXP ELISA Kit allows for the in vitro quantitative determination of Human Pyridoxal phosphate phosphatase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PDXP ELISA Kit has been pre-coated with an PDXP antibody specific to Human Pyridoxal phosphate phosphatase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Pyridoxal phosphate phosphatase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Pyridoxal phosphate phosphatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm