Human permeability glycoprotein ELISA Kit

Product Name:Human permeability glycoprotein ELISA Kit
Packing:96T

Catalog No.:ELA-E1690h

Gene Name:P-gp

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:permeability glycoprotein

Alternative Name:P-gp,permeability glycoprotein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human permeability glycoprotein ELISA Kit allows for the in vitro quantitative determination of permeability glycoprotein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human permeability glycoprotein ELISA Kit has been pre-coated with an permeability glycoprotein antibody specific to permeability glycoprotein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for permeability glycoprotein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain permeability glycoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of permeability glycoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!