Human PFN2 ELISA Kit

Product Name:Human PFN2 ELISA Kit
Packing:96T

Catalog No.:ELA-E0347h

Gene Name:PFN2

Detect Range:0.625-40 ng/mL

Sensitivity:0.33ng/mL

Target Protein Name:Human Profilin-2

Alternative Name:Profilin-2, Profilin II, PFN2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PFN2 ELISA Kit allows for the in vitro quantitative determination of Human Profilin-2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PFN2 ELISA Kit has been pre-coated with an PFN2 antibody specific to Human Profilin-2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Profilin-2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Profilin-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm