PHD3 ELISA KIT|Human
Detect Range 0.156-10ng/ml
PHD3 ELISA KIT Sensitivity< 0.094ng/ml
Application PHD3 ELISA KIT|Human is for detecting the concentration of Human PHD3 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of PHD3 ELISA KIT|Human
The microtiter plate provided in PHD3 ELISA KIT|Human has been pre-coated with an Human PHD3 antibody specific to Human PHD3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human PHD3antibody preparation specific for Human PHD3 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PHD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human PHD3in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that PHD3 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!