Human PHIP ELISA KIT

Catalog  EF001733

Packing 48T/96T                                                 Manual

Reactivity Human

Detect Range 78-5000pg/ml

PHIP ELISA KIT Sensitivity< 39pg/ml

Application Human PHIP ELISA KIT  is for detecting the concentration of Human PHIP in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of Human PHIP ELISA KIT

The microtiter plate provided in Human PHIP ELISA KIT has been pre-coated with an Human PHIP antibody specific to Human PHIP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human PHIPantibody preparation specific for Human PHIP then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PHIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human PHIPin the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that Human PHIP ELISA KIT is only for RUO (Research Use Only), Not for the diagnostic Purpose!