Human Phosphatidylinositol 4- kinase beta, PI4KB ELISA KIT

Product Name:Human Phosphatidylinositol 4- kinase beta, PI4KB ELISA KIT
Packing:96T

Catalog No.:ELI-21557h

Gene Name:PI4KB

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Phosphatidylinositol 4-kinase beta(PI4KB

Alternative Name:PI4KB,Phosphatidylinositol 4-kinase beta(PI4KB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Phosphatidylinositol 4- kinase beta, PI4KB ELISA KIT allows for the in vitro quantitative determination of Phosphatidylinositol 4-kinase beta(PI4KB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Phosphatidylinositol 4- kinase beta, PI4KB ELISA KIT has been pre-coated with an Phosphatidylinositol 4-kinase beta(PI4KB antibody specific to Phosphatidylinositol 4-kinase beta(PI4KB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Phosphatidylinositol 4-kinase beta(PI4KB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phosphatidylinositol 4-kinase beta(PI4KB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Phosphatidylinositol 4-kinase beta(PI4KB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!