Human phospho Protein Kinase C ELISA Kit

Product Name:Human phospho Protein Kinase C ELISA Kit
Packing:96T

Catalog No.:ELA-E0931h

Gene Name:P-PKC

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:phospho Protein Kinase C

Alternative Name:P-PKC,phospho Protein Kinase C
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human phospho Protein Kinase C ELISA Kit allows for the in vitro quantitative determination of phospho Protein Kinase C concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human phospho Protein Kinase C ELISA Kit has been pre-coated with an phospho Protein Kinase C antibody specific to phospho Protein Kinase C .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for phospho Protein Kinase C and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain phospho Protein Kinase C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of phospho Protein Kinase C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!