PHOX2A ELISA KIT|Human
Catalog EF001749
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
PHOX2A ELISA KIT Sensitivity< 0.094ng/ml
Application PHOX2A ELISA KIT|Human is for detecting the concentration of Human PHOX2A in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of PHOX2A ELISA KIT|Human
The microtiter plate provided in PHOX2A ELISA KIT|Human has been pre-coated with an Human PHOX2A antibody specific to Human PHOX2A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human PHOX2Aantibody preparation specific for Human PHOX2A then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PHOX2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human PHOX2Ain the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that PHOX2A ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!