Human PLA2G4A ELISA Kit
Product Name:Human PLA2G4A ELISA Kit
Packing:96T
Catalog No.:ELA-E1624h
Gene Name:PLA2G4A
Detect Range:3.12-200 U/mL
Sensitivity:0.78 U/mL
Target Protein Name:Human Cytosolic phospholipase A2
Alternative Name:Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, PLA2G4A, CPLA2, PLA2G4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human PLA2G4A ELISA Kit allows for the in vitro quantitative determination of Human Cytosolic phospholipase A2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human PLA2G4A ELISA Kit has been pre-coated with an PLA2G4A antibody specific to Human Cytosolic phospholipase A2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Cytosolic phospholipase A2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Cytosolic phospholipase A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm