Human PLCG1 ELISA Kit

Product Name:Human PLCG1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0269h

Gene Name:PLCG1

Detect Range:0.156-10 ng/mL

Sensitivity:0.1ng/mL

Target Protein Name:Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1

Alternative Name:1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PLCG1 ELISA Kit allows for the in vitro quantitative determination of Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PLCG1 ELISA Kit has been pre-coated with an PLCG1 antibody specific to Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm