Human Polymerase I and transcript release factor, PTRF ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PTRF,polymerase I and transcript release factor; RNA polymerase I and transcript release factor; CAVIN; CAVIN1; CGL4; FKSG13; cavin-1; TTF-I interacting peptide 12

Search name

Human PTRF ELISA KIT ,Human polymerase I and transcript release factor ELISA KIT ,Human RNA polymerase I and transcript release factor ELISA KIT ,Human CAVIN ELISA KIT ,Human CAVIN1 ELISA KIT ,Human CGL4 ELISA KIT ,Human FKSG13 ELISA KIT ,Human cavin-1 ELISA KIT ,Human TTF-I interacting peptide 12 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination ofHuman Polymerase I and transcript release factor concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase I and transcript release factor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forPolymerase I and transcript release factor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Polymerase I and transcript release factor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Polymerase I and transcript release factor in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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