Human Polypyrimidine tract- binding protein 1,Ptbp1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PTBP1,PYBP,HNRNP-I; HNRNPI; HNRPI; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; pPTB; Polypyrimidine tract-binding protein 1,57 kDa RNA-binding protein PPTB-1; RNA-binding protein; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; polypyrimidine tract binding protein,heterogeneous nuclear ribonucleoprotein I; polypyrimidine tract binding protein 1

Search name

Human PTBP1 ELISA KIT ,Human PYBP ELISA KIT ,Human HNRNP-I ELISA KIT ,Human HNRNPI ELISA KIT ,Human HNRPI ELISA KIT ,Human PTB ELISA KIT ,Human PTB-1 ELISA KIT ,Human PTB-T ELISA KIT ,Human PTB2 ELISA KIT ,Human PTB3 ELISA KIT ,Human PTB4 ELISA KIT ,Human pPTB ELISA KIT ,Human Polypyrimidine tract-binding protein 1 ELISA KIT ,Human 57 kDa RNA-binding protein PPTB-1 ELISA KIT ,Human RNA-binding protein ELISA KIT ,Human heterogeneous nuclear ribonucleoprotein I ELISA KIT ,Human heterogeneous nuclear ribonucleoprotein polypeptide I ELISA KIT ,Human hnRNP I ELISA KIT ,Human polypyrimidine tract binding protein ELISA KIT ,Human heterogeneous nuclear ribonucleoprotein I ELISA KIT ,Human polypyrimidine tract binding protein 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination ofratPTBconcentrations in serum,Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PTB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PTB,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTB in the samples is then determined by comparing the O.D. of the samples to the standard curve.