Human PON3 ELISA Kit

Product Name:Human PON3 ELISA Kit
Packing:96T

Catalog No.:ELA-E10065h

Gene Name:PON3

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Human Serum paraoxonase/lactonase 3

Alternative Name:Serum paraoxonase/lactonase 3, PON3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human PON3 ELISA Kit allows for the in vitro quantitative determination of Human Serum paraoxonase/lactonase 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human PON3 ELISA Kit has been pre-coated with an PON3 antibody specific to Human Serum paraoxonase/lactonase 3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Serum paraoxonase/lactonase 3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Serum paraoxonase/lactonase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Serum paraoxonase/lactonase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!