Human Porphobilinogen deaminase, HMBS ELISA KIT

Product Name:Human Porphobilinogen deaminase, HMBS ELISA KIT
Packing:96T

Catalog No.:ELI-48602h

Gene Name:HMBS

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Porphobilinogen deaminase(HMBS

Alternative Name:HMBS,Porphobilinogen deaminase(HMBS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Porphobilinogen deaminase, HMBS ELISA KIT allows for the in vitro quantitative determination of Porphobilinogen deaminase(HMBS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Porphobilinogen deaminase, HMBS ELISA KIT has been pre-coated with an Porphobilinogen deaminase(HMBS antibody specific to Porphobilinogen deaminase(HMBS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Porphobilinogen deaminase(HMBS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Porphobilinogen deaminase(HMBS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Porphobilinogen deaminase(HMBS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!