PRB2 ELISA KIT|Human
Detect Range 78.125-5000pg/ml
PRB2 ELISA KIT Sensitivity< 46.875pg/ml
Application PRB2 ELISA KIT|Human is for detecting the concentration of Human PRB2 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of PRB2 ELISA KIT|Human
The microtiter plate provided in PRB2 ELISA KIT|Human has been pre-coated with an Human PRB2 antibody specific to Human PRB2 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human PRB2 antibody preparation specific for Human PRB2 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human PRB2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human PRB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that PRB2 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!